LOCUS       EY193987                 447 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01c02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_497178.1| Kinesin-like protein KLP-20 (73.5 kD)
            (klp-20) [Caenorhabditis elegans] gb|AAK68513.1|AC024801_6
            Kinesin-like protein protein 20 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY193987
VERSION     EY193987.1  GI:159498321
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 852 was extended to value 853 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 640
            PolyA tail found inside read...SR being truncated to 598
            Potential full insert read...vec-to-vec length: 519
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcta ttggaacgaa gagattggcg actggcaatt gaaatgcatt gcctataccg
       61 gcaacaatac gaaagacatt cgtcgtgcca acccggatcg ctcttcatcc gctcacaata
      121 aaccacactg ttctcagatg ctgcctttgt tccattcata caccgatttc ctgggaaatt
      181 cagctgcatc gtcattggct tcatctacta acagcaatat cgccctagtt gggcagagtt
      241 tccgtccgaa atcagcagca aaggatagag atgcggctcg tatacgtgca atgctggaat
      301 aattggctaa accgattgaa attaaatgcg ttttttggcc tccataatca taatactctt
      361 tacctagatc tctggtcaaa aacagtttta ttacttaatg ttttaattta atatttacgt
      421 atatgtataa atagtactta atacata
//