LOCUS EY193984 298 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab01b09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to dbj|BAA21483.1| Bm cdc2 [Bombyx mori], mRNA sequence. ACCESSION EY193984 VERSION EY193984.1 GI:159498318 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 298) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 826 was extended to value 827 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 489 PolyA tail found inside read...SR being truncated to 449 Potential full insert read...vec-to-vec length: 370 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..298 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc aaggcatgga cttgctcaag atgatgtttg tctacaatcc gaacggccgc 61 atctccgcca agcaggtgct ccgccatccg ttcttcaagg acctggagaa gagcaaattg 121 cctgcgcccg actacgacgg cacccttgtg cttcccgttt gagaatgaaa tgagcttatg 181 ataattgact gaggatatgc ggatctttgg gtgtgtctat tttattagca atctggtgtt 241 ggtgggttcg cttgtatcga ttattgaaag cagcaaaatg gataaactca actgagca //