LOCUS       EY193977                 414 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab01a12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193977
VERSION     EY193977.1  GI:159498311
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 789 was extended to value 790 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 606
            PolyA tail found inside read...SR being truncated to 565
            Potential full insert read...vec-to-vec length: 486
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaac ccgcaagagc taaccatcga cgaagggatg cgtcaactgg agcaggccac
       61 caagatgttg gacgcaccgt cggaaattgg agctgcccaa acgcacttta gccagtggaa
      121 agctgctgtc agagaggcgg aacgccgtgg cgacgagctc ttggactcgc ttgagaaaat
      181 gtctgcgaaa atggagtcag ccgcgggcag attcgaccaa gagcacatga tgttcattgc
      241 acaaggggaa aaggcggacg caaacgaccg aactgggcaa acagagtcga acaattgaaa
      301 aatctatttt caactatgga tatcgcaaac aatgttcctg tctgtttgtt ggcttcaatt
      361 tttggggatg attttgtgtt ttttacatgg atgttggatg aatttttgaa cgga
//