LOCUS       EY193960                 490 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab00h02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193960
VERSION     EY193960.1  GI:159498294
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 680
            PolyA tail found inside read...SR being truncated to 641
            Potential full insert read...vec-to-vec length: 562
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gcatcgaaga aagaagctga gggagacgag aagcaggaaa aattggagga
       61 aggggaggag ccgaacaaga aggcgaagtt ggacgtcggc acgaatcgcg accaggatga
      121 gcagcagaaa tgagttcgtg ggagcctcgt gattgatggt gttatttaag aaagaagcat
      181 ctctcactcc cctattttat tcaaaaagtg catccaatct actccaacca cacctctata
      241 tatcttagct aaagccatca tcattcgccc attttatcgc gttagattct accaatctcg
      301 ttatcaaacc ttccatccca actatttaaa gcatgccaaa agacgaaaaa tatccctgag
      361 atattcaagt cagataaaat agattcatcc gccaccagaa tttttgttaa ttttgatgga
      421 gaagaacact tcactgttca atactcaata ctactaatat ttggtaatac gaaataaata
      481 tattagcaca
//