LOCUS       EY193956                 473 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab00g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_759896.1| Conserved hypothetical protein [Vibrio
            vulnificus CMCP6] ref|NP_759902.1| Conserved hypothetical protein
            [Vibrio vulnificus CMCP6] ref|NP_759940.1| Conserved hypothetical
            protein [Vibrio vulnificus CMCP6] ref|NP_760020.1| Orf122-l, mRNA
            sequence.
ACCESSION   EY193956
VERSION     EY193956.1  GI:159498290
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 813 was extended to value 814 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 651
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc agcagtatta agctaaatta ttcttattaa tccaaccttt taggttggat
       61 tctaaatttg agagtttgat cctggctcag aatgaacgct ggcggcaggc ctaacacatg
      121 caagtcgaac gaggttgtat tatagcttgc tgtatacaac ctagtggcag acgggtgagt
      181 aatgtatagg aatctaccta gtagtacgga ataattattg gaaacagtaa ctaataccgt
      241 atacgcccta cgggggaaag atttattgct attagatgag cctatattag attagctagt
      301 tggtagggta atggcttacc aaggctatga tctatagctg atttgagagg atgatcagcc
      361 acactggaac tgagatacgg tccagactcc tacgggaggc agcagtgggg aataatggac
      421 aatgggcgaa agcctgatcc agccatgccg catgagggaa aaaaaaaaaa aaa
//