LOCUS EY193948 254 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00f09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193948 VERSION EY193948.1 GI:159498282 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 254) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 779 was extended to value 780 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 449 PolyA tail found inside read...SR being truncated to 409 Potential full insert read...vec-to-vec length: 326 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..254 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa cggcaaattc tgcaatgacc cgggggccga catcacaatc gtcaactagt 61 cgggcggtag agagggaaac gagaagaact gttgcgtatt agaggcatat gcagttgcaa 121 aaacaaaaca ctgattaaat gcaaatgaac agttgcacaa gagtccccga attgacaaaa 181 tcgggaactt cttctccctc atttgtacaa ttactttttg cctcattttt gcaaaaatat 241 tagcattggt gata //