LOCUS       EY193944                 325 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab00f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193944
VERSION     EY193944.1  GI:159498278
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 518
            PolyA tail found inside read...SR being truncated to 476
            Potential full insert read...vec-to-vec length: 397
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg cgaactgaag gggaccagaa aagcgttgac agaaagccca gtctggtcga
       61 gagtctcgca aaattcggga aattgttcgg actgggcgac cacgagcaga agctctccac
      121 tagtgtggcg caggttctct ctgtctacga cagagcggtg ccacaggcgc gcgaaaaaat
      181 gagggaaaat tccattccta tcgcttcttt tttttaattg ggctgttttc agttttgttg
      241 gggtcatttc cactatttag tttttacaca aatttcatgt gttgccaatc attttcgagt
      301 tcttccgcat aaagtgcatt aaaca
//