LOCUS EY193932 323 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193932 VERSION EY193932.1 GI:159498440 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 323) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 760 was extended to value 761 (,) putative full insert read: vector-to-vector length is 368 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 518 PolyA tail found inside read...SR being truncated to 474 Potential full insert read...vec-to-vec length: 395 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..323 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt ttttgtaatt atgattttag gttataaata attaatgaag tataaactat 61 taatgttata ttttttagat aaattaattt tctagtaatt tatttttaga taaattaatg 121 ttccagaaat atcggctaga cattattatt tttaactaaa aactttttaa attttatttt 181 aatttatata aatttatatt aatataggtg aaattttaat tataattatg ttaataattt 241 tataaaattt aaaattttaa tttttaactt aggttagaca ctaattaatg aaatttaata 301 attttcttta gtaaattttt gta //