LOCUS EY193931 353 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00d08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193931 VERSION EY193931.1 GI:159498439 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 353) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 822 was extended to value 823 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 590 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..353 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg accgcttccg cgtcgcagag ttcgagcagt agtgcgtcta ctccggccat 61 tggggcggcg gtgcaggctg gcgatgacga ggactcgcgc actccgatgc aaaaacgatt 121 gcgtcgaaag ctgtgaagtc gatcttggtg atgacacgtt tatttggatt tgaatgtgga 181 caacatgtgt ttatgggttt cggaccacca actggaaaca aagaaaaatt tatttgagtg 241 atctttgcag ccacttgata ttttccactg gataatatat atgccctccc ctctactcta 301 gtaacagtgt gtattttatt tactttttca aagttcacca gtgtttttgc ttc //