LOCUS EY193930 481 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00d07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_491282.1| Calponin homology (CH) domain containing protein family member [Caenorhabditis elegans] pir|T29467 hypothetical protein F28H1.2 - Caenorhabditis elegans gb|AAB52338.1| Calponin protein 3 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193930 VERSION EY193930.1 GI:159498438 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 481) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 838 was extended to value 838 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 733 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..481 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag cggcgagaat atcagcacca acggcgaccg tgagaacttc tacaaattgc 61 taaaagacgg cacactactg tgcaagctgg ccaacgggat tcaggcgggc tccgtcaaga 121 agatccagca gccgactggc aacttcgcct gcatggagaa catcaacgct ttcgtgtcgt 181 tcgcgaagag ccagggagtg cccaccgagg agaccttcca gagcgtggac ttgttcgagg 241 cacgtgacct ctactccgtg tgcatgaccc tgttgtcgtt gggccgaatt atggagaaga 301 agggaaagcc gaacccattc tctggatgaa gagtagaagt gagtgcagca aaagacggac 361 agagcatgtg ctatcgctcc cattcgagaa ctccccgttt tgcgaatttt ctccccgtgt 421 gcgaccttgc aacaataccg aggcgttaac tgttttcccc tcttctctcc tctcaaactg 481 t //