LOCUS EY193929 383 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193929 VERSION EY193929.1 GI:159498437 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 383) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 805 was extended to value 806 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 660 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..383 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggca gaaggaactt gtggccactt cccaagtgct caagaagacg ggccagttca 61 agtacgtggt ggagctggac cagggtccag tgctgccgga agcaatcgaa cagctggtcg 121 tctttgcgcg caaatactct cgggacaacg gccacttcca gctgcaggtg agtggacgca 181 gccagtgcca cggggtcaac gaggcactgt ccgaactggc ggctgaggat caacaagtgc 241 aacctctgtc taattttgcg gagctggact ataacgagca ttgcgagcct ttcagctgga 301 tttaaaagcg agggcaaacg aaggaacaaa aagtctagtt attcaacatt tatgtactaa 361 tgtttggcaa catccatgtg ttg //