LOCUS EY193927 405 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_491099.1| Actin related protein 2 3 complex p16 [Caenorhabditis elegans] pir|T33169 hypothetical protein M01B12.3 - Caenorhabditis elegans gb|AAC17561.1| Arp2/3 complex component protein 7 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193927 VERSION EY193927.1 GI:159498435 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 405) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 602 PolyA tail found inside read...SR being truncated to 556 Potential full insert read...vec-to-vec length: 477 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..405 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggctg gaaacgaaga ataaggctct tcgggacaaa gcgaccgctt tggttacgcg 61 ggttttgacc agctttaaga atgcagagat gggggcagca gtaaaggcac tctccgctga 121 cgaggtggat ctgctgatga agtacatcta caagggcatg gggttgcttc cggatggaca 181 aacttgcgcc tcactactcg catggcatgc acaggccttt caaatttgcg gacatggcgg 241 cattgtacga gtttatacgg cacgaaatgg actttgaatg tgcaacgcaa tggatcgctc 301 atgtctaatg ccctttactt aatcaaactt ttcaatttgg ttcacttctt gctttattag 361 tcatttcttg ttttataata tatcgaatga aatcctttta aacga //