LOCUS EY193925 466 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00c12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_491349.1| Mannose-1-phosphate guanyltransferase (43.9 kD) [Caenorhabditis elegans] gb|AAF60647.1| Hypothetical protein Y47D9A.1a [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193925 VERSION EY193925.1 GI:159498433 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 466) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 808 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..466 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcac ggccagataa tgttcgagaa ggacctgttc ccgcaactga ccgcgtggag 61 gactctgttc gcgctgcaca cctcccgctg gtggtcccag accaaaaccg ccggtgcggt 121 gctgtacgcg aaccgacact acttgcgcct ctaccgcaag cagaacccgc agctattggc 181 cgaccaccag ctgtgcgaaa tagtcggcga cgtgtacatc gaccccagtg cccaggtgca 241 cccgaccgcc aagataggtc caaacgtgag cattggagcg aatgcgcaga tcaaagcggg 301 agtgcgtgtt cgcgagtcga ttgtgctgca agagtgcgtg ctggacgaga actgctgtgt 361 gctgcacagc gtcatcggct ggcggagcta catcggacct tgggctcgca tcgagggctc 421 ccccatttcg ccgaacccga acattccctt cgccaaactg gacaac //