LOCUS EY193924 397 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00c09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193924 VERSION EY193924.1 GI:159498432 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 397) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 846 was extended to value 847 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 591 PolyA tail found inside read...SR being truncated to 547 Potential full insert read...vec-to-vec length: 469 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..397 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggt ttgttaagat gagcaatcct gcagcacttt tagtattcaa gcggattgtt 61 cacgagttga aacgtgcgga taagaatttt tctacgcaac atccgatcta caaatatttg 121 ttcgatcagc tgcggacgca tcaaatcacg cagcgcatgg cgctcaggca gccaaatgaa 181 ctccagcata tagcaaacag ttacgctgtc tatttggaga gtagtcggcg gttagcggag 241 cttcatcaaa aatactatgc cagcggcgaa agatctgtgg aggaaacagc gcgcattgtt 301 gggctcaaag tgccagcaat taacgcggac tgatggaaaa tacgaaaata gtctttgatc 361 gctgatttta taaatttcac acacaatttt atgatta //