LOCUS EY193920 288 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00c03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193920 VERSION EY193920.1 GI:159498428 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 288) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 861 was extended to value 861 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 481 PolyA tail found inside read...SR being truncated to 439 Potential full insert read...vec-to-vec length: 360 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..288 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg gacacagatt cggatgggac gatctcgccc agcgaggcac ttgcgttctg 61 gaaggcaaag gcggagacga tggatccaga ggagctgaag aagcggctgg agaagttcaa 121 cgcaaagacc ctggaggaag cggtggaccg cggcttcaag aaggtggaca aggactcgga 181 cgggaaagtg agcccggaag aattgaaggc gtacatacag agctgattat attctcgacc 241 acttgacgat gttacgcttt ccctgctcca aataaacacg catccaca //