LOCUS EY193912 250 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00b04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193912 VERSION EY193912.1 GI:159498420 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 250) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 750 was extended to value 751 (,) This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 440 PolyA tail found inside read...SR being truncated to 400 Potential full insert read...vec-to-vec length: 322 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..250 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa agaaacaaac aaggagaaga gagataacta attttgttcg tcgacattcg 61 aaatcccttt atttttttaa ctggatgacc cattcctcag tttcttgcag tcacataaag 121 cgatatagga tagaatatat tacatatttt tcgtttgtga tagattttgc acccatccgc 181 taatatctcc ccgaccatgg caaataaaaa gtatcctgga tcacaaaaaa aaaaaaaaaa 241 aaaaaaaaga //