LOCUS EY193911 335 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00b02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193911 VERSION EY193911.1 GI:159498419 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 335) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 526 PolyA tail found inside read...SR being truncated to 486 Potential full insert read...vec-to-vec length: 407 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..335 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat tctggcggaa gcggaagatc catcagcatt cgcccgacaa tgttcctcca 61 aagcgacgac acagtgccca ccggcgccgt tctgctcgct gaacgtgtcg cccgaggaaa 121 gcagggacga gctgagaaga gtgctcaatg aatgaaaacg cggatgagga aggaagcgtt 181 aaaaatatgg tcaaataaat gtgcacacaa accaattcaa ataaatccat cgcgacttta 241 ctttatgatt ctggctcttt tcgcaccaca ccagttcgtg ctttcgttct ttctataatc 301 tatacattga gacaaagaat aaaaacaata tcgca //