LOCUS EY193910 255 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00a12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193910 VERSION EY193910.1 GI:159498418 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 255) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 430 was extended to value 430 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 438 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..255 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa gccgtgcagg cgttccgacc gcaagctatt tggctccgaa agtgaagtgt 61 caattgagtc agtgggaact gggaacggac atcatcgtgt gtggaggaga tgtgagatgc 121 cgcagttgtg taataaaaat gttcggcaaa ttgcgtgcaa tgaataaaca caaaaaagat 181 ccctcatccg cttgtcgccc tcaacttgtc cccgctcatt tagccccccc atttgtccgt 241 cgtccgccgt gtcgc //