LOCUS EY193909 256 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00a10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193909 VERSION EY193909.1 GI:159498417 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 256) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 822 was extended to value 823 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 450 PolyA tail found inside read...SR being truncated to 407 Potential full insert read...vec-to-vec length: 328 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..256 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtag ataaattaat ttttctagta atttattttt agataaatta atgttccaga 61 aatatcggct agacattatt atttttaact aaaaactttt taaattttat tttaatttat 121 ataaatttat attaatatag gtgaaatttt aattataatt atgttaataa ttttataaaa 181 tttaaaattt taatttttaa cttaggttag acactaatta atgaaattta ataattttct 241 ttagtaaatt tttaca //