LOCUS EY193907 324 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00a08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to pir|T30896 hypothetical protein T08B1.2 - Caenorhabditis elegans, mRNA sequence. ACCESSION EY193907 VERSION EY193907.1 GI:159498415 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 324) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 785 was extended to value 786 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 517 PolyA tail found inside read...SR being truncated to 476 Potential full insert read...vec-to-vec length: 396 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..324 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat aggcaaatcg accgccgcaa tttcaaagag cgaagggcaa tttacgacaa 61 aaaaaatgcg tacccatgct tcccaaatgt tcctcctcct ccggttattc tagaatttaa 121 gccacttcca gaaatcggag gaggcgatgc cagaggggca gattacgaag gcgacgaaga 181 cgaggaggag tgaagagtga tcggtgatcg catacgcagg acaacatttg tcaaaccgaa 241 catgagaata ctcgattaat tagttggaca aaattatgtt atttttctac catgatttta 301 accaataatt actaattaga taga //