LOCUS EY193905 373 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00a06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193905 VERSION EY193905.1 GI:159498413 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 373) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 810 was extended to value 810 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 567 PolyA tail found inside read...SR being truncated to 524 Potential full insert read...vec-to-vec length: 445 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..373 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggct actacggagt gtatcggcca gcttgctcca ttcttcggct gctcttcgcg 61 ctcagctatt acgcgataat ggtggccggc atttggttca tcttcggtcc gctcttcgac 121 ccatatgtac cactgcctgg attcgcgcga aatgtgtcgt tgtccgcgat caactcagcc 181 agatgggaag tgagcagcac tgtaaggact gcgttagaat ccgtcaaatt ggtgatcaag 241 tttacggcca aactcattgt ccgccttttg gagtgatgcc ggtttgcttc agtgggtgaa 301 tttatttgct tttcggcaca tctccacaat cacaagtttt tgtgatcatt tgcattcggg 361 taaaacggtc tca //