LOCUS EY193904 278 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00a05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_497827.1| Glyoxalase protein (32.1 kD) [Caenorhabditis elegans] sp|Q09253|YQ5A_CAEEL HYPOTHETICAL 32.0 KD PROTEIN C16C10.10 IN CHROMOSOME III pir|T19331 hypothetical protein C16C10.10 - Caenorhabditis elegans emb|CAA86748.1| Hypothetical p, mRNA sequence. ACCESSION EY193904 VERSION EY193904.1 GI:159498412 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 278) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 828 was extended to value 829 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 469 PolyA tail found inside read...SR being truncated to 430 Potential full insert read...vec-to-vec length: 350 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..278 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga cactccgggg aaggcaactg tgtgcgtcgt catactccga gacccgaatg 61 aacatgaaat ctgctttgtc ggcgatgagg gctttcgcca actgtcgaag gcggacccgg 121 atgcggaaca actgctccag gaggcgatca agaaggatga cagcatgaac tgggcctagg 181 ggaagaagtc atgactggac atgtgtggga cttacttggg cacaactcac ttatggcatt 241 tgggaaatgt gaaatgcatg taaatgcatg ggtcagta //