LOCUS EY193901 181 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab00a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193901 VERSION EY193901.1 GI:159498409 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 181) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 821 was extended to value 821 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 373 PolyA tail found inside read...SR being truncated to 332 Potential full insert read...vec-to-vec length: 253 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..181 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc caatcgctta tcaatttcag ctgaaaaatg aatttgagac cttcttgtaa 61 ttaattatat atcaatcgat taccagctag cacagaagac ttctgggcgg ccactctatt 121 ctccaactct gccaatcact catcaatttc agctgataaa tgaataaatg aatttgagac 181 a //