LOCUS EY193900 239 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99h11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193900 VERSION EY193900.1 GI:159498408 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 239) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 155 original value before phred recall for SR was 437 PolyA tail found inside read...SR being truncated to 395 Potential full insert read...vec-to-vec length: 353 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..239 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac acgagaatat tccgataatg tttgctctgt tcaatatcca acaacaaccg 61 agtttatctg gcccgcttgt aatgtggctg gacacgatct cctttatcaa aatcacatcc 121 cactactctt tcgtccatca tgcccttcgg ataaaaatga cttgtaatct aaaaaaccag 181 taatgggatt gtgtttttat taaatggcct caaacaatta aaatacattc gcacctcca //