LOCUS EY193899 460 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99h10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193899 VERSION EY193899.1 GI:159498407 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 460) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 655 PolyA tail found inside read...SR being truncated to 614 Potential full insert read...vec-to-vec length: 596 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..460 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcga gagaatggat cagaagcaac aggaacagct cgaaatatgt ctcaatgccc 61 tcatcagcac aatcgaagat tgcgagttgg aacgcgaaga gaactgggaa cgcctaaagc 121 ggttcgttaa gttcgcaagt atagctgctg ttggcgccgt gctggtgttc ctcagtgttc 181 tctattggcc ggttatcgca ccattggcag gacgagtggt gcgctcgatt tgggaactga 241 tttccagcct tgtggacctg gtcaaagcgt acttgcccga aattatcggc ggcactttgg 301 cgctaaccat tttaatggac ccaatgtgcc gctcgcttgg cggagaaact cagcagaggg 361 aataaaacat gaatatgctg caaaatcact tggttgtcat tttataaggc agccaatgtt 421 cattaattcg ttatcatgca ataaatattt acgaatttga //