LOCUS EY193898 375 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99h09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193898 VERSION EY193898.1 GI:159498406 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 375) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 571 PolyA tail found inside read...SR being truncated to 529 Potential full insert read...vec-to-vec length: 513 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..375 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcag cagtaatcta tttgaccttg ataacctact tggataactg tggtaattct 61 agagctaata catgcacaaa aactcccaac gcaaggaggg agcgcatttg ttcgccacaa 121 aaccaagccc cctcgggggt ggagtgttga atcagagcaa ctaagctgag cgcacggtct 181 tgcaccggcg ccgtatcatt cgagcttctg ccctatcaac tttcgacggt agtgtctctg 241 gctaccgtgg tggtgacggg taacggagga tcagggttcg actccggaga aggggcctga 301 gaaatggcca ctatgtctaa ggatagcagc aggcgcgcaa attacccact ctcagcatga 361 ggaggtagtg acgga //