LOCUS       EY193897                 273 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa99h08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY193897
VERSION     EY193897.1  GI:159498405
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 819 was extended to value 819 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 469
            PolyA tail found inside read...SR being truncated to 427
            Potential full insert read...vec-to-vec length: 411
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat agcaataccg ttgctccccg gtgtcgggag gcccagtgta tgtgttccgc
       61 aggaatacag cccaatgccc cagcatctgg cccggcgcgc tcgcttaact gtgggcgcgt
      121 tgccaaagcc gtagcactcg tgtgccatgg tggaacgtcg gggcgcaaga tcacttgtat
      181 acgacttgac tactggtcag ggggttgtta gcagtagagc agcctttatg ctgcgatctg
      241 ttgagactca gcccttgatc ggatgatttg tga
//