LOCUS EY193894 609 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99h05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_506352.1| Transthyretin-like family member precursor family member (17.8 kD) [Caenorhabditis elegans] pir|T20267 hypothetical protein C56A3.2 - Caenorhabditis elegans emb|CAB01136.1| Hypothetical protein C56A3.2 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY193894 VERSION EY193894.1 GI:159498402 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 609) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 820 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..609 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa tttgagatgt caatttcgaa gaattacctg gcatttgttc tccttttgtg 61 cttctgttcc cttggctcag cgatcaggac tcagtctgct gctgtaaaag gtcgcttgat 121 gtgtggtgcg gcaccggcga gaaatgttcg agtcaaactg ttcgatgagg attctggacc 181 cgaccctgac gaccaattag acgcaggcta cacggactca aatgggaact tccagctgaa 241 gggctccacc accgaagcaa ccaacatcga cgtcgtgttc aagatctacc acgactgcga 301 cgatgggatt aagcctggct ctcgcaaaat caagttcaag attcccaact cgtacgtcag 361 tgagggagcc acccccaaaa aaacatttga catcggcacg ctgaatctgg agaccatctt 421 cgccaacgag gagagggagt tgatcgtgag caagaaaagg cgacacttgc accactaccg 481 gggcggcgag aaattcaact ttttcgttcg tctctaggtg gcaaagaaag aagaatttgg 541 ataatggata agccaaaaca aacaaacaat acaaatcgac cctctttcga cttgtcaaat 601 tgcttttta //