LOCUS EY193891 503 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99h02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to emb|CAA09591.1| elongation factor 1-beta [Globodera rostochiensis], mRNA sequence. ACCESSION EY193891 VERSION EY193891.1 GI:159498399 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 503) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 822 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..503 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg aacaacgaga aggagctgaa cgagttcaat gagcacttgt ccacgcgagg 61 atacatccat ggttttcttc catcgcaaga agatcaatac gtgttcaagt gcttcacagc 121 tgcccctgcc caaaaatacg cgcacatcac gcgttggtac aagcacatcc agagctatga 181 agacgcagag cgctcaactt ggccacaaaa agccggtgga cctccttcag ccagtgctgc 241 caaacaatct tctccgccca agggagcagc cgctgccgcc ggcggagatg acgatttcga 301 cctgttcggc gatgaagacg gcgatgacga agagaagaag cgaatcaccg aggagcgctt 361 gaaggcctac gcggagaaga agtccaagaa gcccggcccc atcgccaagt ccaacgtgat 421 ctacgatgtc aagccctggg acgacactgt ggacatcaag gagatcgaga agaatgtgcg 481 ctctattcac acagaggggc ttg //