LOCUS EY193889 540 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193889 VERSION EY193889.1 GI:159498397 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 540) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 713 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..540 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa gtttgagatt cttcttactc ttactgcaaa atatacatgg gagatgggag 61 tagacacgca agatcaagaa gcattggtaa cggagttgag agcaaaaata gtcgaacttg 121 aacagactgt caaaaagcaa gatgaagaaa tcttagagct gaaaatgttg agcatcgtag 181 acaaggaatt gttggaggtg caaaaggaaa ccgagaagga actcaaagac caactggacc 241 aggaagctat aaccatctcc cagcttgaat atcaacttga agaaaaggac aaaatgactg 301 acaagcttga gtttgccttg atcaagttcc gtgagaaaat cgacgagctc aacgaccaaa 361 ttaaacatca acaagaggag ctcaacggac aaatgagtgc tgagcaaatg atttccgagt 421 tgaccagcaa aaatctggac ttggaagaac aaatgaaatt atacgagaca acaattgccg 481 aactggagga aatgcaccaa ttaaacgaag cgattattga ggcgaaaaaa aaaaaaaaaa //