LOCUS EY193888 638 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_500424.1| Zinc finger plus homeodomain, Axon Guidance ZAG-1, Zinc-finger homeodomain protein (zag-1) [Caenorhabditis elegans] pir|T29204 hypothetical protein F28F9.1 - Caenorhabditis elegans, mRNA sequence. ACCESSION EY193888 VERSION EY193888.1 GI:159498396 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 638) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 827 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..638 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggcg gcgcaatcaa acatcagccg gcggcaaagc gaagacgcac tgtccagtcg 61 ggagaagaag ccggtggaac agcggtggcc atcgttggcc cctacatttg cgatctgtgc 121 gaaaagtcgt tcaacaaaca gagctcgttg gcacgccaca aatatgagca ttcgggccaa 181 cgcccataca agtgccaaat gtgcgaaaag gcgttcaagc acaagcacca cctgacggag 241 cacaaccgac tgcacagtgg cgagaagccg ttccagtgca acaaatgccg aaaaaggttc 301 tcccattccg gctcctactc ccagcacatg aaccatcgat acgcctattg caagccgttt 361 atgacgggaa aagctggcgg aagtgactcc cccgctgggg aacggacaac gcccagtgtt 421 gaagagtcgc ccaatgagag ggaggaaagt tgtgacaacg gcactattgt tgaggggaac 481 ggaccaattg aaatgggcga agaagtcaag tgtgagccgg gagagctcaa ttgtgatctg 541 agttgattgg agactgaaca ttgagagaac ttcaaatccc atccttcaat ttacaatttt 601 gcttcatcaa agattccttt gccatcctca tttctgtc //