LOCUS EY193887 550 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193887 VERSION EY193887.1 GI:159498395 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 550) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 824 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..550 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac agacgcattc ttcggaaagg aactgatcga acgcccaaaa tgcaactctc 61 tatttccgtt gtttccctgg ttctcttggg cgcggttgca gccgtccaat cggcccccac 121 gggatttgtg ctcccgaacc aggcggagat cgagagcgcc ttccgctcga agcggaacat 181 tccctacgag gacacgtgtc cggcggaaga ggtgatcccg ctgatcgacg ccatctcgga 241 cgtggccaag gcgaagagcg acggccgcgg ggtgcagatc gcggtgctgg agtacttcct 301 gaagaagaag accggctgca tctggggcgt gatcatctcg cagccgggcc aagagaccga 361 ctgagagatc cccgctctgt accgcggcaa gaccacgcac cacgcccagt tctgcaacca 421 cgtgcacaac gaccagttct acagcgtgtt catgatggac tgcgaccagc caaagccaca 481 cgagccgacc acgactgaat ctctgcagtc gctggaactt gccacagacg caccaactgg 541 gaactgagct //