LOCUS EY193886 599 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_187574.1| 60S ribosomal protein L4/L1 (RPL4A) [Arabidopsis thaliana] sp|Q9SF40|RL4B_ARATH 60S ribosomal protein L4-2 (L1) gb|AAF23293.1|AC016661_18 putative 60S ribosomal protein L1 [Arabidopsis thaliana] gb|AAL09727.1| AT3g09630/F11F8_22 [, mRNA sequence. ACCESSION EY193886 VERSION EY193886.1 GI:159498394 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 599) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 619 (,) original QR value of 810 was extended to value 811 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 768 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..599 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggacc gtcgttacaa gcaaaagctc ggaccgctgt tcatttacaa caacgatggg 61 gggattgttc gggccttccg caacattcct ggggtcacac tgctctctgt caaccgcatc 121 aatttgctga agattgctcc tggtggacat ttgggacgtc tcgttgtgtg gaccgagagt 181 gctttccgca aattggattc tatcttcggc agctttacca ggaaatctga tgttaagaag 241 gggttcactc tgccccgcgc aaaaatgacc aactccgacc tcgtgcgtat cattcgctcg 301 gacgaaattc agaaggttgt gcgtccgatc aagaagcagg ggaaggtcgc aaaggttcac 361 cgcaatccgc tgaagaagca ctctttgatg gtcaaactga acccctatgc ttccgttcaa 421 cgcagagccg caattatcgc gtcggctaag gggcaacagc aaaaggccta gaagaatgaa 481 ttgagtgggt accggacaaa catcagctgg cggaaaattc gaatgttgac catttttgtt 541 ggcaaaattg ttatttcatg ttgcaaataa actgcacgga caaaaaaaaa aaaaaaaaa //