LOCUS EY193885 571 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193885 VERSION EY193885.1 GI:159498393 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 571) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 822 was extended to value 822 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 828 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..571 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac tcttgtatcg gcaaaaaacg ttttgctctt tagactacag aaatctggat 61 aaaccaaaac catgggtgtg ccaatgatcc aggtgttttt gattttggct cgccaaatgt 121 caaaaccaat aagcgacaga ataatgggat tcggtaggaa gcatccgtat gttcgcaatc 181 atattttaat tcccatcgga acaggaataa caaatttttc tacacggcta cgattgaaga 241 acttgggttt gacgaaacca acccaatcgg caactgttag cgaggaagtg gcgcttgaac 301 aggcgtcaga gacgatccag caggctgtaa tcttctttta ctccgtttcg ctttacgcct 361 tatacaatta ctattcgaag aaaggcgaaa aagaaactgc cacgcgagaa gaaattgtgg 421 atctcttgga aaaggtcaag aagttggaga cgaacaagtt gtggatggaa gagcggatat 481 tgaggttgga agaacatctg gcgaaaaagg gaggcagcag cggtttgctt aattttatgc 541 caaaagtcgg aaagctggaa ctgtaccgac a //