LOCUS EY193884 220 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|P47904|RS27_XENLA 40S ribosomal protein S27 pir|S35758 ribosomal protein S27, cytosolic - African clawed frog emb|CAA50485.1| ribosomal protein S27 homologue [Xenopus laevis], mRNA sequence. ACCESSION EY193884 VERSION EY193884.1 GI:159498392 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 220) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 829 was extended to value 830 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 417 PolyA tail found inside read...SR being truncated to 374 Potential full insert read...vec-to-vec length: 292 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..220 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc accaccgtgt tcagccatgc gcagacagtc gtggtgtgcg tcggatgcag 61 caccgtgctg tgccagccga ccggcggaaa ggcgcgtctg gtggagggat gctcgctcag 121 aaagaagcag cactgaacag aagatggacg tatctggttt gtcgaactgt ttttgttgat 181 cggtaatttg gacggcgcca caatatatat ttgttgtcca //