LOCUS EY193882 548 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193882 VERSION EY193882.1 GI:159498390 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 548) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 817 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..548 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa ttgggcaaaa ttagcggagc gattgacacc agcgcaagaa gctgagctga 61 gcaagttgaa gttgcggaac ccaaacttcc atgcagagat agttcaactt caagccgatc 121 cgccaaaagt ggattggacc gcattgaaga agcaaatgcc tgagagttca gcggcgctgg 181 atgcattgca aaaacaatgg gggtcgttga gcattccata tgggagtgtg cccgaaccgt 241 tgagtaaaca agttgaccag tggatacaat acaatgaagc cagaaccaag tttcatgcac 301 aaaagagcgc agacggggaa gaggaggcga aaaaaattga tgctaaatgg gctaagtgtc 361 caccggttga gcacatgcaa cctgatcagt ttgtgcaata ttttggcgga gataaaaagt 421 tcgctttgtt ccgtcgtcac atggatttcc gctatgaagt cgatgaattc catgacgttt 481 ggggcaccgg tgtgaacgaa cgaggcacct acaaggaacg cttcgccgat tacagagttg 541 gccgccgt //