LOCUS EY193881 561 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99g02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193881 VERSION EY193881.1 GI:159498389 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 561) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 805 was extended to value 805 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 809 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..561 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtca caatcaaaag cagggaattg atgctaacac atttgataaa ttgagcgatc 61 cgcgaataaa agagcaactt gtccagtccc tggccgatgg aactgtgtac tcgattttgg 121 attcgctcaa ggatttgcaa tcactccaag aatttaatct atggcaacag cgggagcaag 181 agctggaaca gttaaaacgg attgaccatc cagacattgc cgaaaagacg cgcgaggtgg 241 atcaacgaat tttggagcgg attgaccaga tagcctatga acagcagaat acgctgacct 301 gcgccggagt tccgatgttc aagccaagcg aggaaggggc tgacctacgc gcccaaatgg 361 ccattatacg cttcattctt tcgctggaat ccatctttag tcacacagaa tcatcattta 421 accagagtgg gcaagaaggc tgaatcggta gaccaattat tacccaaata ctgtgtataa 481 tgacaaaaca tcaatttcat cttttctatg gaaccataat aattttatac attggcctga 541 ccccagcttg ttagtattaa a //