LOCUS EY193875 607 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193875 VERSION EY193875.1 GI:159498383 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 607) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 834 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..607 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac agtcttgttt agttcgcctc tgaagatgat gctgcgctcg tgtgtgctcg 61 tcactctggt ctttggcgcg ctgtgttctg ctgtccgagc ccagcaagtg aagcaatgcc 121 tgtgctccga gatcactccc tgcaaggacg cccatttgaa cgcgctggag ccgtgcatgg 181 actcatgcca gcaccatctg gcctcgatgg gcggcaacta ccagcagctg aagacgtgtc 241 tgaagcagcg ccagaacctg ctcacgtccg ccatcagctg cactgagggc caattttcca 301 acgcgtgagt gggacggcgc aattaaggca aaactttgtt ttctagctgt gcaaactccc 361 ccggacaaat ggtgccgaaa cgttatccgg aaacactgca aatcgctaca attagcgaga 421 tcaaccgcat gatcaacaag atggggctgt ccggtcaggc gaagggactg atggacgcag 481 gcaagaagat cttcggctgc atgaaaactt gcatggccaa aaaatcgggc aactgcgaca 541 agaacttgaa gtgcggactg gcactgcccc cggacaatgt ggtggtgcag attgtcaaga 601 agtgcgc //