LOCUS       EY193871                 342 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa99f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_495807.1| Initiation Factor Five eIF-5A homolog
            IFF-2 (18.0 kD) (iff-2) [Caenorhabditis elegans]
            sp|Q20751|IF52_CAEEL Eukaryotic translation initiation factor 5A-2
            (eIF-5A-2) pir|T22628 translation initiation factor eIF-5A F54C9.1
            [similari, mRNA sequence.
ACCESSION   EY193871
VERSION     EY193871.1  GI:159498379
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 830 was extended to value 830 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 536
            PolyA tail found inside read...SR being truncated to 493
            Potential full insert read...vec-to-vec length: 414
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg accagataaa acaggcgtac gagaaagacg agaacggcat acttgtcaat
       61 gtggtgtccg cttgcggcga ggaagctatt ctcgggtgga agtacatgcc aaatcgcgaa
      121 taattgttgc ccacggcagg cacgggagag acgaggatga gtttgggtga aaatggagga
      181 ggatggtcga cgtaaaagac agcattcgat caacaaattc tcctctctct cgtccaattt
      241 ccattttgtt gttgttatat caaatcgacg ccaccgactt tcagattcaa tcataaattc
      301 atcctctgtt cctctcctat gcttaaagat gttgactgga ca
//