LOCUS EY193870 286 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|P48155|RS7_MANSE 40S RIBOSOMAL PROTEIN S7 gb|AAA20402.1| ribosomal protein s7 [Manduca sexta], mRNA sequence. ACCESSION EY193870 VERSION EY193870.1 GI:159498378 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 286) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 482 PolyA tail found inside read...SR being truncated to 439 Potential full insert read...vec-to-vec length: 358 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..286 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc cgcgctcccg aacactcacc tccgtgcatg aggctatcct gtcggacatt 61 gtcctccccg cggaagtcgt tggcaagcgg acccgcgtaa agctggatgg aaaacaactc 121 atcaaggtcc acctggacaa atcgcagcaa accaacgttg agcacaaagt ggacactttt 181 tctcagcttt acaaacggat gaccgggaag gaagttacgt tcgaattccc cgagccactg 241 ttctaaacaa tgacaaggag atggactcat aaatttgttt ttgcca //