LOCUS EY193868 588 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa99e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY193868 VERSION EY193868.1 GI:159498376 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 588) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 614 (,) original QR value of 808 was extended to value 808 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 762 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..588 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt ttttgtaatt atgattttag gttataaata attaatgaag tataaactat 61 taatgttata ttttttagat aaattaattt ttctagtaat ttatttttag ataaattaat 121 gttccagaaa tatcggctag acattattat ttttaactaa aaacttttta aattttattt 181 taatttatat aaatttatat taatataggt gaaattttaa ttataattat gttaataatt 241 ttatgaaatt taaaatttta atttttaact taggttagac actaattaat gaaatttaat 301 aattttcttt agtaaatttt tgtaaaaaaa gaaaaaatta aggcagaaat attaagaatt 361 tttggagttt gaagattaat tagagaaacc tctttattat ctttatttat tgtttcttgt 421 atgtttatcg taaaattaat tattttattt tttattaata tttttatata aaattagtag 481 ataaatatcg ggattataat ggagtttttt ttaactacaa atattataaa tttttatttg 541 aatattatag taattatata ttgaaatagg aaaaaaaaaa aaaaaaaa //