LOCUS       CO897661                 489 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04g01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element TAR1 repetitive element, mRNA
            sequence.
ACCESSION   CO897661
VERSION     CO897661.1  GI:51237451
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgacggcgga gaggagctct ccagacatct gataaaccga ttgaaaaagg aaaaaggatg
       61 tgaagagaag gcaaagctca gggcggagtt ggcgcaggcc gaagagcgta gcaagagagc
      121 caaggaacga agcgagattg ccaaggaacg tagcaagaga gccaaggaac gaagcgagat
      181 tgccaaggaa cgtagcaaga gagccaagga acgaagcgag attgccaagg aacgtagcaa
      241 gagagccaag gaacgaagcg agattgccaa ggaacgtagc aagagagcta aggaacgtat
      301 cgaaatcgct aacgctaaaa ttgccgagct tagccgccaa ttggaggagt tgcgcaacat
      361 gcaggcccac agaatgaatg acgtcgcgtc tgctggctga agtgtttttc tcgatcaatc
      421 agttaactgt tccttcaaaa ttgttttgta agccaacaat ctctgattta tccgttatat
      481 catcccctc
//