LOCUS       CO897660                 570 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04f12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YMS2_CAEEL P34498 PUTATIVE PRE-MRNA SPLICING
            FACTOR ATP-DEPENDENT RNA HELICASE K03H1.2. [1], mRNA sequence.
ACCESSION   CO897660
VERSION     CO897660.1  GI:51237450
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 545.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agttctaagt tctctataaa aatgcaatcg atggatgatg acgcttcggt ttccgtattg
       61 gagggaacaa gtggtagaga accgggtgga ttgattttca tgaaaaatcc aagcaaatgc
      121 aaagattcat cagtgaagga cgacgacaaa ttgttcaaaa agcccacttc aatacttggt
      181 cttgacaggc ttgctaggca aaagcgcgaa gaaaatttac gcaaaaggca agctgaatca
      241 ggtgatgaaa cccccggact gaccgatagc acaagacgcg agatcacaca atatatagcg
      301 gaaaaatatc gcgatgacag agatcggcgt ggaatcgatg ccggccttaa agaggagaga
      361 aagagatgcg acagagatcg agatagaagg gatagaaatc ggcgccatcg atttgacaga
      421 aacaggcctt catggaaaga tgtcgatacg cctaaattca aattgccaaa cactccttct
      481 gcatcacaat gggacgagga agatttcccg gggcagaggc ctccctccaa aaagtctgct
      541 tgggactttt aaacgccaaa aaaagaaaaa
//