LOCUS       CO897657                 542 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04f09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9UAX1 Q9UAX1 T12B3.4 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897657
VERSION     CO897657.1  GI:51237447
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 450.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttaatgcac ggaaatgctc gccaaagaca tggccaacag cttcttcgat tttctgcacg
       61 ttaacaaaca aaagacattt cggccaaaga agcggttcca gcatggcaca ctgcgctaca
      121 atctgcacaa gcaggcccag gccacactgc actcgggcgt tgacctgcga caggcggtca
      181 agctgccaaa gggcgaaacc ctggacgact ggctggcagt gaacaccgtg gacttcttca
      241 accgggtgaa cctgctgtac ggcattctgg ccgacctgtg cactgcggag acgtgtccga
      301 cgatgagcgg agggccgagg tacgagtacc tgtggcagga cggactggag ttcaagaagc
      361 ccacaaggct gccggcaaag gactacatgc acaaactgat ggactggatc gaggagcgca
      421 tcaacgacga gcgccagttc ccctccagca cggaagtgcc ctttccaccg gaattccgtc
      481 agacctgcaa gaacaccagg tcgcggcagg taaggatctt agaggaatta gtaatcccgc
      541 aa
//