LOCUS       CO897655                 564 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04f07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE21666 Y39B6A.F, mRNA sequence.
ACCESSION   CO897655
VERSION     CO897655.1  GI:51237445
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 550.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 attcgctttt cttggcatta ttcaatagat taaaaaatga ccactcggca attcaataaa
       61 ttgtacctga tgccaccaaa tcgcaaaacc cgcatggctg aaacgatgga cataatattc
      121 gggcgaaaag aggaagaaaa aagagtccaa ctcgatttat tgaatcgaga agtgaagcgc
      181 agtaaaccgc ggcgagaaga tcaatcattt tctcaggatt ggcccagtgt gtggccagct
      241 gcgcagtcat ttcgctcttc cgtggtacca attccggttc gaatgggtta ccgcaagaac
      301 ccggaaaaaa aagccccggc agctgcatat gccaatctgg agctcgcaaa aatggccaat
      361 tttctgcact tgacacctgc acatgtccaa cgtcactgcg aggccatcaa aaagttttgt
      421 accaagtttc ccgaggaatt gaaggagcgc gaatgcgaag ctcgctactt cccgctacgc
      481 taccattacg acacatatgt acatcaggga aatagcattc gtgatatgcg cgcgcgcgtc
      541 gtcacagtca cgcctgaggt tgga
//