LOCUS       CO897582                 424 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03e10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO897582
VERSION     CO897582.1  GI:51237372
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgaaatgga gtttaagaat ggtgaacgga gcggtagggc cgacgcaacg tggacggagg
       61 tgcacgaagc ggtgtatgca ctgctctaca agaactacct ggtcgggatg cacgatacgt
      121 ttgaaatcgt gcacgaagtc tatcgcttta acggcatctg cctcatcgac tttgcgtcca
      181 agcatggctt tgcctctttc gaagagatgc tgcattcggt caagatggaa aacgtggtcg
      241 taattgaaga ggtccagaca ccagaaacca agcgccgata ctttcttcca atgaagtacc
      301 gccatcgccc caaactgagc gcagaatttg cgcaaacgcg tgagacaagc tgcgccaccg
      361 aaattgattc tcggactcct aacagaggaa gcgccctcgt ccacgctgtc cgaggcggac
      421 tctg
//