LOCUS       CO897553                 538 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03b07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YOT9_CAEEL P34654 HYPOTHETICAL 13.4 KD PROTEIN
            ZK632.9 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO897553
VERSION     CO897553.1  GI:51237343
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 573
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtcttcatac ccaaaccacg atggcatcaa accaatccgc cgccgaactg gccgaggagt
       61 acgagcgtgg agagcacgac gaagtcccca agagtggcca gggcaagcgc tccaaggcag
      121 aacaggtgga gcacaagcac gcgcacccgg agggggacac gcgtcgcatc acggacaatg
      181 cggtgaaggg cgaggagaaa cgcaaggagg aggagcgcaa gcgcaccaac tcccagtcca
      241 agtgagcggc ggaggagaac gacggaagcg tgtgtgtgtg ccttgttgaa taacgtgacc
      301 gatgaaaaca actcgagccc ttcaaaaaaa caattcactc ctttctagtg aacttccttc
      361 actttcattt tcgagatatt ccccgcacac gctgacggcc aacaaattcc atctttccac
      421 aaaaacaatc ctcgatccac acacagattt tgttaagcag aagaataaag agccattgtg
      481 aaccggcaca caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//