LOCUS       CO897549                 475 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03a12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O45499 O45499 F39B2.6 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897549
VERSION     CO897549.1  GI:51237339
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aacggccgtg gacacgtcca gcccattcgc tgcaccaact gcggacgctc ctgccccaag
       61 gacaaggcga tcaagaaatt tgtggtccgc aacattgtgg aggcggccgc agttcgcgac
      121 atcactgatg cttcgattta cgaacaatac gctctgccga agctgtacca caagctgcac
      181 tactgtgttt cgtgcgcaat tcacagcaag gtggtgcgca acagatcgcg tgaggctcgt
      241 aaggaccgaa acccgccgcc gcgcttcgga cagcgtgcgg ccgctgaccg cccgcctcgt
      301 cctggagcac cagccgccgg aggtccgggc gctggagctg ggggtccgcc agcaattcgg
      361 ggatagacgg tatacaattg gagcacacaa agaagctaag agacggcaaa aatcccccga
      421 ttgttgatgt tttcattgtt atttttgcaa tttggcaaag ctgattggtt gaaac
//