LOCUS       CO897547                 595 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03a10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL24_CAEEL Q17606 PROBABLE 60S RIBOSOMAL PROTEIN
            L24. [1], mRNA sequence.
ACCESSION   CO897547
VERSION     CO897547.1  GI:51237337
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 604
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtgcgagaa atgaggctgg agaatgctgg ttctgctcct cgacatatat cccggacacg
       61 gcatccagtt tgtccgaaat gatggaagta tcttcaagtt ctgtcgctcc aaatgcaaca
      121 aattgttcaa gaaaaagaag aatccgcgga agctcaggtg gacgaaagcc tcacgccggc
      181 tacgcggaaa agagttggtc aacgatgaaa ctcaagcgat ggaagtgcgc cggaatgaac
      241 cgaccaaata cgaacgagcg ctctggaaag atgcagtcga gacaatcaag gatattcaca
      301 caatcaaaga gcgccgctac ggacaacaca ttgcgaatac gctgaagccg ggtaaacaaa
      361 ttgcgaagag ggcaatgctg cagaaggcga gaaagaaagt gcatctgatc agagcgccag
      421 tggcagatga ccaagaagag gaagaggtgg agactgagga ggatgaagga aagccggaac
      481 tggaggaagg tgaagcgcag atggaggtca attaagcaaa gtgattttgt ttcttgttta
      541 acatttccgc gataaatcgt aactggtcaa aaaaaaaaaa aaaaaaaaaa acaaa
//