LOCUS       CO897544                 542 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03a05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:CYC1_ASCSU P92504 CYTOCHROME C, TYPE-1. [1], mRNA
            sequence.
ACCESSION   CO897544
VERSION     CO897544.1  GI:51237334
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 548
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gctttttgct gagactgcca atggccgaaa ttcctgaggg tgactacgag aagggaaaga
       61 aagtcttcaa ggcccggtgt ctccaatgcc acgtgatcga ctccatggcc acaaagacag
      121 gaccaaccct gaatgggatt tggggaagga aaagtggaca ggtacccgga ttcgactatt
      181 ctgccgccaa caaaaataag ggtgttgttt ggaatcgcga aacgctcttc gaatacctgc
      241 tcaatccaaa gaaatatatc ccggggacga agatggtttt tgcagggctg aagaaggtcg
      301 acgaaagggc ggatctcatc aagtacatcg aggttgaaag tcagaaaccg ccagcatgat
      361 gaattgacgc atgagaggtt catcgcccgt ccacgacaaa caggatagtt tatagtcagt
      421 cacgtgcgag acacttggtt aaaattgttt ttcacctaga cattttcaca ctctgaggcc
      481 ctcgtttcta agaaattgtt agttcgaaaa aataaaattc gttgacaaaa aaaaaaaaca
      541 aa
//